Porsolt’s newly developed Real Time PCR (qPCR) capabilities can be used to detect key molecular pathway modulations at early stages and quantitate changes in gene or micro-RNA expression in response to different environmental conditions and/or drug treatments. This rapid and sensitive analysis can be performed on different types of biological samples from various animal species and enhances the translational value of preclinical studies. Porsolt’s scientists design the oligonucleotides based on the specific needs of our clients, and these are used in multiplex qPCR reactions to obtain the most information from the study samples.
This technique enhances the value and provides a more comprehensive picture pre-clinical, investigative, safety assessment, and toxicology studies, and can be incorporated as part of studies being performed at Porsolt or as a standalone service for clients interested in sending preclinical and clinical samples for analysis.
Below are examples of results from the Unilateral Ureteral Obstruction (UUO) model of renal disease where we quantified markers of fibrosis and inflammation (CTL: non-operated, UUO: UUO kidney).
Please do not hesitate to contact us (contact@porsolt.com) to discuss incorporating this service as part of studies performed at Porsolt, or to independently analyze stored tissue samples or frozen cells.
Find out more about all our capabilities at www.porsolt.com
